Archive for the ‘Transfection Books’ Category
Last Updated on Tuesday, 6 July 2010 11:29 Written by Administrator Tuesday, 6 July 2010 11:29
Gene Transfer and Expression Protocols (Methods in Molecular Biology)
Roche Products Ltd, Welwyn Garden City, Hertfordshire, U.K.. Techniques for introducing an isolated gene into a cell line using a variety of transfection techniques. For final year graduate students, postgraduates, or postdoctorate scientists in gene regulation research.
List Price: $ 114.50
Price: $ 98.73
Tags: Biology., Expression, gene, methods, Molecular, Protocols, Transfer | Posted under Transfection Books | No Comments
Last Updated on Monday, 5 July 2010 04:02 Written by Administrator Monday, 5 July 2010 04:02
Generation of a retroviral vector that expresses an anti-HIV-1 tat hammerhead ribozyme.(Clinical report): An article from: Journal of the South Carolina Academy of Science
This digital document is an article from Journal of the South Carolina Academy of Science, published by South Carolina Academy of Science on September 22, 2009. The length of the article is 3271 words. The page length shown above is based on a typical 300-word page. The article is delivered in HTML format and is available immediately after purchase. You can view it with any web browser.
From the author: Ribozymes have emerged as promising therapeutics for HIV and have been shown to bind and cleave target RNA’s in a sequence-specific manner. The HIV-1 regulatory protein tat plays an essential role in the upregulation of viral transcription and elongation of viral transcripts, and has therefore been identified as a target for ribozyme studies. To further study the use of these reagents, we have designed a number of hammerhead ribozymes targeted to specific sequences within the HIV-1 NL43 tat sequence. One of these, Tat5910 and its non-catalytic control Tat5910A, were cloned into the retroviral vector pSuper.retro.neo+GFP (pSRNG) for cell culture studies. Recombinant retrovirus particles were generated by transient transfection of 293T cells using a two-plasmid system consisting of the helper plasmid, pPAM3, and pSRNG5910A. Identical experiments were carried out using two Mo-MLV retroviral vectors, pLNCE, and pLNCLZRz. The resulting recombinant virus was used to transduce NIH-3T3 cells, and virus titer (virus particles/ml) was determined from the number of Green Fluorescent Protein (GFP)- or P-galactosidase-positive cells. Although producer cells transfected with pSRNG5910A expressed GFP, the transfection efficieny was low. This resulted in levels of transduced NIH-3T3 cells that were too low to obtain a reliable titer measurement. Attempts to optimize titer by increasing the number of transfected producer 293T cells was successful using pLNCE and pLNCLZRz. Positive GFP or P-galactosidase expression in NIH-3T3 cells transduced with LNCE and LNCLzRz recombinant virus indicated that our two-plasmid virus production system was successful. Extremely low titer along with lower cellular expression of GFP suggests that the pSRNG5910 vectors may be better suited to virus production using a stable producer cell line such as PA317
Citation Details
Title: Generation of a retroviral vector that expresses an anti-HIV-1 tat hammerhead ribozyme.(Clinical report)
Author: Lindsey E. Padgett
Publication: Journal of the South Carolina Academy of Science (Magazine/Journal)
Date: September 22, 2009
Publisher: South Carolina Academy of Science
Volume: 7 Issue: 2 Page: 18(5)
Article Type: Clinical report
Distributed by Gale, a part of Cengage Learning
List Price: $ 9.95
Price: $ 9.95
Tags: Academy, antiHIV1, article, Carolina, expresses, from, Generation, hammerhead, Journal, report, retroviral, ribozyme.Clinical, Science, South, vector | Posted under Transfection Books | No Comments
Last Updated on Saturday, 3 July 2010 05:46 Written by Administrator Saturday, 3 July 2010 05:46
Extratropical Transition of Western North Pacific Tropical Cyclones
This is a NAVAL POSTGRADUATE SCHOOL MONTEREY CA report procured by the Pentagon and made available for public release. It has been reproduced in the best form available to the Pentagon. It is not spiral-bound, but rather assembled with Velobinding in a soft, white linen cover. The Storming Media report number is A024143. The abstract provided by the Pentagon follows: Extratropical transition (ET) of a tropical cyclone (TC) often results in a mid-latitude storm that threatens maritime and coastal interests. Cases of ET between 1 July through 31 October during 1994-1996 are reviewed using Navy Operational Global Atmospheric Prediction System (NOGAPS) analyses and hourly geostationary satellite imagery. Current conceptual models are found to be inadequate to explain the physical processes in ET. ET is redefined to have two stages: transformation, where the TC is transformed from a warm-core vortex into a baroclinic, cold-core extratropical cyclone, and re- intensification, where the transformed TC either deepens or dissipates, depending on the existence of upper4ropospheric support for extratropical cyclogenesis. ET is further defined in terms of two characteristic mid-latitude synoptic patterns: meridional, in which the cyclones have meridional tracks and tend to re-intensify less vigorously than zonal, which have zonal tracks and may deepen explosively. Review of NOGAPS 5OO-mb anomaly correlation scores in 1996 demonstrated that ET may be associated with significant NOGAPS errors. Sea-level pressure forecasts during ET events involving a merger tend to be too deep. In ET cases of rapidly deepening storms, NOGAPS tends to overforecast their intensity during transformation, and then underforecast during re- intensification. Rules of thumb are provided to assist forecasters in improving predictions of the track and intensity of storms undergoing ET.
Price: $ 33.95
Tags: Cyclones, Extratropical, North, Pacific, Transition, Tropical, Western | Posted under Transfection Books | No Comments
Last Updated on Thursday, 1 July 2010 06:37 Written by Administrator Thursday, 1 July 2010 06:37
A Comparative Analysis of the Iridium and Globalstar Satellite Transmission Paths
This is a AIR FORCE INST OF TECH WRIGHT-PATTERSONAFB OH report procured by the Pentagon and made available for public release. It has been reproduced in the best form available to the Pentagon. It is not spiral-bound, but rather assembled with Velobinding in a soft, white linen cover. The Storming Media report number is A217163. The abstract provided by the Pentagon follows: Globalstar and Iridium will provide valuable global communications assets for business, humanitarian aid and military operations. However, the level of coverage and the quality of the transmission path of these systems are strongly dependent on the latitude of the user and, due to their orbital characteristics, both systems provide reduced levels of coverage at low latitudes. Additionally, the L- and S-Band frequencies utilized by these systems are prone to ionospheric interference at low latitudes. The results of extensive simulation analysis indicate that the Globalstar constellation architecture provides a considerably better transmission path than Iridium’s in several important areas, including path elevation angles, satellite visibility and susceptibility to ionospheric effects. To assist future study, a unique set of equations has been developed which describe the distribution of Iridium and Globalstar path elevation angles entirely as a function of the user’s latitude. In addition to the differences in path elevation angles, modeling indicates that ionospheric scintillation is a potentially serious problem for both systems. However, Globalstar is expected to suffer lower fade levels than Iridium due to its higher downlink frequency and multiple path availability. The research concludes that, within the scope of the analysis, Globalstar provides a higher quality transmission path for low to mid-latitude users.
Price: $ 48.95
Tags: analysis, comparative, Globalstar, Iridium, Paths, Satellite, Transmission | Posted under Transfection Books | No Comments