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	<title>Cell Transfection &#187; Transfection Questions</title>
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	<link>http://www.celltransfection.com</link>
	<description>Cell Transfection</description>
	<lastBuildDate>Sat, 07 Aug 2010 19:35:40 +0000</lastBuildDate>
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		<title>Transgenic cells are created via the _______ of recombinant DNA into cells.?</title>
		<link>http://www.celltransfection.com/transgenic-cells-are-created-via-the-_______-of-recombinant-dna-into-cells-182/</link>
		<comments>http://www.celltransfection.com/transgenic-cells-are-created-via-the-_______-of-recombinant-dna-into-cells-182/#comments</comments>
		<pubDate>Sat, 07 Aug 2010 19:35:40 +0000</pubDate>
		<dc:creator>Administrator</dc:creator>
				<category><![CDATA[Transfection Questions]]></category>
		<category><![CDATA[cells]]></category>
		<category><![CDATA[created]]></category>
		<category><![CDATA[into]]></category>
		<category><![CDATA[recombinant]]></category>
		<category><![CDATA[Transgenic]]></category>
		<category><![CDATA[_______]]></category>

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		<description><![CDATA[









Question by deadyetpresent: Transgenic cells are created via the _______ of recombinant DNA into cells.?
A)recombination
B)transfection
C)ligation
D)restriction
E)complementation
Best answer:
Give your answer to this question below!



]]></description>
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		</item>
		<item>
		<title>Hi, does anyone know how many weeks/months is the eluted DNA stable in a Tris-Cl,EDTA, pH8 solution at 4dgr?</title>
		<link>http://www.celltransfection.com/hi-does-anyone-know-how-many-weeksmonths-is-the-eluted-dna-stable-in-a-tris-cledta-ph8-solution-at-4dgr-181/</link>
		<comments>http://www.celltransfection.com/hi-does-anyone-know-how-many-weeksmonths-is-the-eluted-dna-stable-in-a-tris-cledta-ph8-solution-at-4dgr-181/#comments</comments>
		<pubDate>Sat, 07 Aug 2010 19:35:39 +0000</pubDate>
		<dc:creator>Administrator</dc:creator>
				<category><![CDATA[Transfection Questions]]></category>
		<category><![CDATA[4dgr]]></category>
		<category><![CDATA[anyone]]></category>
		<category><![CDATA[eluted]]></category>
		<category><![CDATA[know]]></category>
		<category><![CDATA[many]]></category>
		<category><![CDATA[solution]]></category>
		<category><![CDATA[Stable]]></category>
		<category><![CDATA[TrisClEDTA]]></category>
		<category><![CDATA[weeks/months]]></category>

		<guid isPermaLink="false">http://www.celltransfection.com/hi-does-anyone-know-how-many-weeksmonths-is-the-eluted-dna-stable-in-a-tris-cledta-ph8-solution-at-4dgr-181/</guid>
		<description><![CDATA[Question by AndroG: Hi, does anyone know how many weeks/months is the eluted DNA stable in a Tris-Cl,EDTA, pH8 solution at 4dgr?
To be more precized, I extracted the plasmid with my gene of interest from a bacteria with the special maxi kit endo-free from Qiagen and I eluted it with a buffer which contains 10mM [...]]]></description>
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		<slash:comments>0</slash:comments>
		</item>
		<item>
		<title>Does anyone know how the Gene Jammer Transfection Reagent works?</title>
		<link>http://www.celltransfection.com/does-anyone-know-how-the-gene-jammer-transfection-reagent-works-89/</link>
		<comments>http://www.celltransfection.com/does-anyone-know-how-the-gene-jammer-transfection-reagent-works-89/#comments</comments>
		<pubDate>Fri, 25 Jun 2010 04:37:00 +0000</pubDate>
		<dc:creator>Administrator</dc:creator>
				<category><![CDATA[Transfection Questions]]></category>
		<category><![CDATA[anyone]]></category>
		<category><![CDATA[gene]]></category>
		<category><![CDATA[Jammer]]></category>
		<category><![CDATA[know]]></category>
		<category><![CDATA[Reagent]]></category>
		<category><![CDATA[Transfection]]></category>
		<category><![CDATA[works]]></category>

		<guid isPermaLink="false">http://www.celltransfection.com/does-anyone-know-how-the-gene-jammer-transfection-reagent-works-89/</guid>
		<description><![CDATA[Question by Kokk Kostas: Does anyone know how the Gene Jammer Transfection Reagent works?
We used GeneJammer Transfection Reagent in cells, before adding DNA. But I don&#8217;t know what it does exactly.
Best answer:
What do you think? Answer below!
]]></description>
		<wfw:commentRss>http://www.celltransfection.com/does-anyone-know-how-the-gene-jammer-transfection-reagent-works-89/feed/</wfw:commentRss>
		<slash:comments>0</slash:comments>
		</item>
		<item>
		<title>What is a RIPA analysis in cell transfection studies?</title>
		<link>http://www.celltransfection.com/what-is-a-ripa-analysis-in-cell-transfection-studies-80/</link>
		<comments>http://www.celltransfection.com/what-is-a-ripa-analysis-in-cell-transfection-studies-80/#comments</comments>
		<pubDate>Thu, 17 Jun 2010 00:10:35 +0000</pubDate>
		<dc:creator>Administrator</dc:creator>
				<category><![CDATA[Transfection Questions]]></category>
		<category><![CDATA[analysis]]></category>
		<category><![CDATA[cell]]></category>
		<category><![CDATA[RIPA]]></category>
		<category><![CDATA[studies]]></category>
		<category><![CDATA[Transfection]]></category>

		<guid isPermaLink="false">http://www.celltransfection.com/what-is-a-ripa-analysis-in-cell-transfection-studies-80/</guid>
		<description><![CDATA[Question by sam_kh918: What is a RIPA analysis in cell transfection studies?
Best answer:
Add your own answer in the comments!
]]></description>
		<wfw:commentRss>http://www.celltransfection.com/what-is-a-ripa-analysis-in-cell-transfection-studies-80/feed/</wfw:commentRss>
		<slash:comments>0</slash:comments>
		</item>
		<item>
		<title>Does anyone know of a good method for transfection of HEK 293 cells?</title>
		<link>http://www.celltransfection.com/does-anyone-know-of-a-good-method-for-transfection-of-hek-293-cells-77/</link>
		<comments>http://www.celltransfection.com/does-anyone-know-of-a-good-method-for-transfection-of-hek-293-cells-77/#comments</comments>
		<pubDate>Tue, 15 Jun 2010 23:51:54 +0000</pubDate>
		<dc:creator>Administrator</dc:creator>
				<category><![CDATA[Transfection Questions]]></category>
		<category><![CDATA[anyone]]></category>
		<category><![CDATA[cells]]></category>
		<category><![CDATA[good]]></category>
		<category><![CDATA[know]]></category>
		<category><![CDATA[method]]></category>
		<category><![CDATA[Transfection]]></category>

		<guid isPermaLink="false">http://www.celltransfection.com/does-anyone-know-of-a-good-method-for-transfection-of-hek-293-cells-77/</guid>
		<description><![CDATA[Question by Scooter: Does anyone know of a good method for transfection of HEK 293 cells?
I have been transfecting HEK 293 cells with Lipofectamine and I lose 90-100% of the cells after transfection. I&#8217;m trying to find a way to transfect and get the results I want with minimal cell death.
Best answer:
Give your answer to [...]]]></description>
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		<slash:comments>0</slash:comments>
		</item>
		<item>
		<title>Why does transfection efficiency increase when the amount of DNA added increases in cells?</title>
		<link>http://www.celltransfection.com/why-does-transfection-efficiency-increase-when-the-amount-of-dna-added-increases-in-cells-85/</link>
		<comments>http://www.celltransfection.com/why-does-transfection-efficiency-increase-when-the-amount-of-dna-added-increases-in-cells-85/#comments</comments>
		<pubDate>Fri, 11 Jun 2010 13:45:43 +0000</pubDate>
		<dc:creator>Administrator</dc:creator>
				<category><![CDATA[Transfection Questions]]></category>
		<category><![CDATA[added]]></category>
		<category><![CDATA[amount]]></category>
		<category><![CDATA[cells]]></category>
		<category><![CDATA[Efficiency]]></category>
		<category><![CDATA[increase]]></category>
		<category><![CDATA[increases]]></category>
		<category><![CDATA[Transfection]]></category>

		<guid isPermaLink="false">http://www.celltransfection.com/why-does-transfection-efficiency-increase-when-the-amount-of-dna-added-increases-in-cells-85/</guid>
		<description><![CDATA[Question by mjorizzle: Why does transfection efficiency increase when the amount of DNA added increases in cells?
Best answer:
Give your answer to this question below!
]]></description>
		<wfw:commentRss>http://www.celltransfection.com/why-does-transfection-efficiency-increase-when-the-amount-of-dna-added-increases-in-cells-85/feed/</wfw:commentRss>
		<slash:comments>0</slash:comments>
		</item>
		<item>
		<title>What is the proper technique to gain a stabily transfected cell line using Lipofectamine 2000 on HeLa cells?</title>
		<link>http://www.celltransfection.com/what-is-the-proper-technique-to-gain-a-stabily-transfected-cell-line-using-lipofectamine-2000-on-hela-cells-64/</link>
		<comments>http://www.celltransfection.com/what-is-the-proper-technique-to-gain-a-stabily-transfected-cell-line-using-lipofectamine-2000-on-hela-cells-64/#comments</comments>
		<pubDate>Thu, 03 Jun 2010 15:25:14 +0000</pubDate>
		<dc:creator>Administrator</dc:creator>
				<category><![CDATA[Transfection Questions]]></category>
		<category><![CDATA[2000]]></category>
		<category><![CDATA[cell]]></category>
		<category><![CDATA[cells]]></category>
		<category><![CDATA[gain]]></category>
		<category><![CDATA[HeLa]]></category>
		<category><![CDATA[line]]></category>
		<category><![CDATA[Lipofectamine]]></category>
		<category><![CDATA[proper]]></category>
		<category><![CDATA[stabily]]></category>
		<category><![CDATA[technique]]></category>
		<category><![CDATA[transfected]]></category>
		<category><![CDATA[using]]></category>

		<guid isPermaLink="false">http://www.celltransfection.com/what-is-the-proper-technique-to-gain-a-stabily-transfected-cell-line-using-lipofectamine-2000-on-hela-cells-64/</guid>
		<description><![CDATA[Question by benji: What is the proper technique to gain a stabily transfected cell line using Lipofectamine 2000 on HeLa cells?
I know the general scheme, but wanted more specifics of how long to treat with reagent of choice and when to split when selecting.
Best answer:
Know better? Leave your own answer in the comments!
]]></description>
		<wfw:commentRss>http://www.celltransfection.com/what-is-the-proper-technique-to-gain-a-stabily-transfected-cell-line-using-lipofectamine-2000-on-hela-cells-64/feed/</wfw:commentRss>
		<slash:comments>0</slash:comments>
		</item>
		<item>
		<title>In vivo DNA transfection?</title>
		<link>http://www.celltransfection.com/in-vivo-dna-transfection-61/</link>
		<comments>http://www.celltransfection.com/in-vivo-dna-transfection-61/#comments</comments>
		<pubDate>Tue, 01 Jun 2010 19:43:13 +0000</pubDate>
		<dc:creator>Administrator</dc:creator>
				<category><![CDATA[Transfection Questions]]></category>
		<category><![CDATA[Transfection]]></category>
		<category><![CDATA[vivo]]></category>

		<guid isPermaLink="false">http://www.celltransfection.com/in-vivo-dna-transfection-61/</guid>
		<description><![CDATA[Question by Think.: In vivo DNA transfection?
Every time I hear about transfection, it refers to in vitro transfection. Is there any way to transfect DNA into cells in a living organism?
Best answer:
Know better? Leave your own answer in the comments!
]]></description>
		<wfw:commentRss>http://www.celltransfection.com/in-vivo-dna-transfection-61/feed/</wfw:commentRss>
		<slash:comments>0</slash:comments>
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