Archive for the ‘Transfection Questions’ Category
Last Updated on Tuesday, 15 June 2010 11:51 Written by Administrator Tuesday, 15 June 2010 11:51
Question by Scooter: Does anyone know of a good method for transfection of HEK 293 cells?
I have been transfecting HEK 293 cells with Lipofectamine and I lose 90-100% of the cells after transfection. I’m trying to find a way to transfect and get the results I want with minimal cell death.
Best answer:
Give your answer to this question below!
Tags: anyone, cells, good, know, method, Transfection | Posted under Transfection Questions | No Comments
Last Updated on Friday, 11 June 2010 01:45 Written by Administrator Friday, 11 June 2010 01:45
Question by mjorizzle: Why does transfection efficiency increase when the amount of DNA added increases in cells?
Best answer:
Give your answer to this question below!
Tags: added, amount, cells, Efficiency, increase, increases, Transfection | Posted under Transfection Questions | No Comments
Last Updated on Thursday, 3 June 2010 03:25 Written by Administrator Thursday, 3 June 2010 03:25
Question by benji: What is the proper technique to gain a stabily transfected cell line using Lipofectamine 2000 on HeLa cells?
I know the general scheme, but wanted more specifics of how long to treat with reagent of choice and when to split when selecting.
Best answer:
Know better? Leave your own answer in the comments!
Tags: 2000, cell, cells, gain, HeLa, line, Lipofectamine, proper, stabily, technique, transfected, using | Posted under Transfection Questions | No Comments
Last Updated on Tuesday, 1 June 2010 07:43 Written by Administrator Tuesday, 1 June 2010 07:43
Question by Think.: In vivo DNA transfection?
Every time I hear about transfection, it refers to in vitro transfection. Is there any way to transfect DNA into cells in a living organism?
Best answer:
Know better? Leave your own answer in the comments!
Tags: Transfection, vivo | Posted under Transfection Questions | No Comments