Posts Tagged ‘Animal’
Last Updated on Thursday, 17 June 2010 11:40 Written by Administrator Thursday, 17 June 2010 11:40
Gene Transfer to Animal Cells (Advanced Methods)
The Adanced Methods series is intended for advanced undergraduates, graduate students and established research scientists. Titles in the series are designed to cover current important areas of research in life sciences, and include both theoretical background and detailed protocols. The aim is to give researchers sufficient theory, supported by references, to take the given protocols and adapt them to their particular experimental systems.
Gene transfer to animal cells was first achieved more than 30 years ago. Since then, transformation technology has developed rapidly, resulting in a multitude of techniques for cell transformation and the creation of transgenic animals. As with any expanding technology, it becomes difficult to keep track of all the developments and to find a concise and comprehensive source of information that explains all the underlying principles. Gene Transfer to Animals Cells describes the principles behind gene transfer technologies, how gene expression is controlled in animal cells and how advanced strategies can be used to add, exchange or delete sequences from animal genomes in a conditional manner. The final chapter provides an overview of all the applications of animal cell transformation in farming, medicine and research.
Rating: 
(out of 1 reviews)
List Price: $ 81.00
Price: $ 59.32
Last Updated on Thursday, 10 June 2010 01:08 Written by Administrator Thursday, 10 June 2010 01:08
Cell Culture: Animal, Tissue culture, Organ culture, Laboratory, Passaging, Transfection, Transformation (genetics), Microbiological culture, Viral culture
Cell culture is the process by which cells are grown under controlled conditions. In practice the term “cell culture” has come to refer to the culturing of cells derived from multicellular eukaryotes, especially ? animal cells. The historical development and methods of cell culture are closely interrelated to those of ? tissue culture and ? organ culture. Animal cell culture became a common ? laboratory technique in the mid-1900s,[1] but the concept of maintaining live cell lines separated from their original tissue source was discovered in the 19th century.
List Price: $ 51.00
Price: $ 47.20
Tags: Animal, cell, Culture, genetics, Laboratory, Microbiological, Organ, Passaging, Tissue, Transfection, Transformation, Viral | Posted under Transfection Books | No Comments
Last Updated on Wednesday, 9 June 2010 12:59 Written by Administrator Wednesday, 9 June 2010 12:59
Transient Transfection in Animal Cell Culture: A comparative study of different methods for transient transfection in suspension adapted Chinese Hamster Ovary cells
The transient gene expression (TGE) in mammalian cells is an attractive option to rapidly produce research grade material with high quality that, for instance, can streamline the identification of candidate proteins in clinical trials. In this thesis we report on the performance of different methods to transiently transfect mammalian cells, in particular Chinese Hamster Ovary (CHO) cells. The used transfection methods can be categorized into physical and chemical. We compared the feasibility, the efficiency (both cost efficiency and transfection efficiency) and suitability regarding to up-scale the procedure. The optimization of the different transfection methods was carried out using statistical approaches. In this work, the successful optimization of the transient transfection and the up-scale into the bioreactor are reported. Furthermore a good insight of the different transfection methods and their parameters were obtained, which can ease the application of TGE.
List Price: $ 66.00
Price: $ 64.20
Tags: adapted, Animal, cell, cells, Chinese, comparative, Culture, different, Hamster, methods, Ovary, study, suspension, Transfection, Transient | Posted under Transfection Books | No Comments