Posts Tagged ‘anyone’
Last Updated on Saturday, 7 August 2010 07:35 Written by Administrator Saturday, 7 August 2010 07:35
Question by AndroG: Hi, does anyone know how many weeks/months is the eluted DNA stable in a Tris-Cl,EDTA, pH8 solution at 4dgr?
To be more precized, I extracted the plasmid with my gene of interest from a bacteria with the special maxi kit endo-free from Qiagen and I eluted it with a buffer which contains 10mM Tris-C,pH8.0 and 1mM EDTA.I want to know how long can I store the eluted DNA at 4 degrees before using it for transfection into cells. Is DNA stable for several weeks or months? I would appreciate very much your answer as I am just a PhD student with not so much experience in molecular biology. Thank u very much.
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Tags: 4dgr, anyone, eluted, know, many, solution, Stable, TrisClEDTA, weeks/months | Posted under Transfection Questions | No Comments
Last Updated on Friday, 25 June 2010 04:37 Written by Administrator Friday, 25 June 2010 04:37
Question by Kokk Kostas: Does anyone know how the Gene Jammer Transfection Reagent works?
We used GeneJammer Transfection Reagent in cells, before adding DNA. But I don’t know what it does exactly.
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Tags: anyone, gene, Jammer, know, Reagent, Transfection, works | Posted under Transfection Questions | No Comments
Last Updated on Tuesday, 15 June 2010 11:51 Written by Administrator Tuesday, 15 June 2010 11:51
Question by Scooter: Does anyone know of a good method for transfection of HEK 293 cells?
I have been transfecting HEK 293 cells with Lipofectamine and I lose 90-100% of the cells after transfection. I’m trying to find a way to transfect and get the results I want with minimal cell death.
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Tags: anyone, cells, good, know, method, Transfection | Posted under Transfection Questions | No Comments