Posts Tagged ‘cells’
Last Updated on Wednesday, 16 June 2010 11:01 Written by Administrator Wednesday, 16 June 2010 11:01
Our vision at Aldevron is to be a world-class global contract services organization. To achieve this, we need to understand what our clients need and deliver what they want. One of our services is expression screening in insect cells. We offer a range of options to suite the clients needs. A typical screening project includes multiple variants, such as amino acid changes or truncations, of a single gene. We are able to screen multiple variants and culture conditions in parallel. There are a couple of options for expression screening in insect cells. The first option is to screen for expression via baculovirus infection. We can either start with a recombinant transfer vector supplied by the client or we can clone the gene of interest into a baculovirus transfer vector. Next, we generate a high-titer baculovirus stock and determine the viral titer. We then infect 10ml Sf21 cultures for recombinant protein expression. The second option is to screen for expression via direct transfection. This allows us to go from plasmid to protein in as little as 48 hours. We start by cloning the gene of interest into an expression vector designed to enable robust expression in insect cells without the need to produce recombinant baculovirus. Next we transfect 10ml Sf9 cultures with the recombinant plasmids. 48-72 hours post-transfection, we harvest the cells. Because generating a recombinant baculovirus can require anywhere between 2-4 weeks of time, the direct transfection method offers a …
Tags: Aldevron, cells, Expression, Insect, Protein | Posted under Transfection Videos | No Comments
Last Updated on Tuesday, 15 June 2010 11:51 Written by Administrator Tuesday, 15 June 2010 11:51
Question by Scooter: Does anyone know of a good method for transfection of HEK 293 cells?
I have been transfecting HEK 293 cells with Lipofectamine and I lose 90-100% of the cells after transfection. I’m trying to find a way to transfect and get the results I want with minimal cell death.
Best answer:
Give your answer to this question below!
Tags: anyone, cells, good, know, method, Transfection | Posted under Transfection Questions | No Comments
Last Updated on Sunday, 13 June 2010 07:18 Written by Administrator Sunday, 13 June 2010 07:18
www.cellectricon.com | The Cellaxess®HT system is a revolutionary new concept in high throughput transfection enabling the transfer from “cooperative cell lines” to biologically relevant model systems in large scale genomic screening. The Cellaxess®HT system is the only transfection system on the market enabling highly efficient gene transfer on a wide range of biologically relevant cell types at a throughput of 140.000 wells in 24 hours.
Tags: cDNA, cell, Cellaxess®HT, cells, Delivery, lines, primary, Reagentfree, siRNA, System | Posted under Transfection Videos | No Comments
Last Updated on Saturday, 12 June 2010 06:34 Written by Administrator Saturday, 12 June 2010 06:34
DNA Transfer to Cultured Cells (Culture of Specialized Cells)
DNA transfer to cultured cells
Edited by Katya Ravid and R. Ian Freshney
Rapid advances in DNA transfer technology have transformed many disciplines, ranging from molecular genetics to biotechnology. Scientists now have the means to introduce copies of genes into different cell types, then detect the expression of these genes in the cell. It is now possible to regulate cell growth that may lead to cancer, develop new biopharmaceuticals, and apply knowledge about the role of genes in cell processes to basic research in molecular genetics.
DNA Transfer to Cultured Cells is the first quick reference to all of the established techniques for the transfer of genetic material to cells in vitro. Featuring contributions by leading researchers in the field, this detailed guide walks the reader through a variety of DNA transfer methods, describes their application to specific cell types, and integrates aspects of molecular biology with tissue culture. Offering overviews and detailed protocols for the techniques under discussion in each of its sections, this book covers an exceptionally broad array of topics, including:
* Viral infection
* Electroporation
* Phosphate precipitation
* DEAE Dextran
* Liposomes
* Yeast artificial chromosomes (YACs)
* Whole chromosome transfer
* Enhanced expression.
Special sections at the end of each chapter list suppliers for necessary reagents and materials. This easy-to-use, self-contained guide addresses key developments of recent years as well as emerging trends in DNA transfer. For practical applications in cell biology, genetics, heredity, biotechnology, or evolution, DNA Transfer to Cultured Cells is a unique and unparalleled resource.
List Price: $ 149.95
Price: $ 25.99
Tags: cells, Culture, Cultured, Specialized, Transfer | Posted under Transfection Books | No Comments