Posts Tagged ‘Chinese’
Last Updated on Wednesday, 9 June 2010 12:59 Written by Administrator Wednesday, 9 June 2010 12:59
Transient Transfection in Animal Cell Culture: A comparative study of different methods for transient transfection in suspension adapted Chinese Hamster Ovary cells
The transient gene expression (TGE) in mammalian cells is an attractive option to rapidly produce research grade material with high quality that, for instance, can streamline the identification of candidate proteins in clinical trials. In this thesis we report on the performance of different methods to transiently transfect mammalian cells, in particular Chinese Hamster Ovary (CHO) cells. The used transfection methods can be categorized into physical and chemical. We compared the feasibility, the efficiency (both cost efficiency and transfection efficiency) and suitability regarding to up-scale the procedure. The optimization of the different transfection methods was carried out using statistical approaches. In this work, the successful optimization of the transient transfection and the up-scale into the bioreactor are reported. Furthermore a good insight of the different transfection methods and their parameters were obtained, which can ease the application of TGE.
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Tags: adapted, Animal, cell, cells, Chinese, comparative, Culture, different, Hamster, methods, Ovary, study, suspension, Transfection, Transient | Posted under Transfection Books | No Comments
Last Updated on Sunday, 6 June 2010 11:02 Written by Administrator Sunday, 6 June 2010 11:02
Biopharmaceuticals: A Study of Apoptosis and Cell Cycle to Augment Transfection Efficiency in Chinese hamster ovary (CHO) Cells
In the biopharmaceutical industry, essentially, there are three components that play the main role in producing biopharmaceutical products, the host cell, the expression vector and the bioreactor and/or production environment. To produce the highly valued and desired products, the choice of a suitable host is one of the most important aspects. The host required is not only required to produce the desired product, but also needs to demonstrate robustness in a bioreactor system. Constantly facing challenges in a bioreactor, cells often undergo apoptosis, a well-known limiting factor in biopharmaceutical production, which ultimately leads to low yield of valuable protein(s). We have genetically engineered a CHO-K1 cell line to constitutively express human insulin-like growth factor-1 (IGF-1) and murine polyoma large T-antigen (PyLT-Ag) to generate Super-CHO and CHO-T respectively, two cell lines that can potentially serve different niches in the biopharmaceutical industry.
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Tags: Apoptosis, Augment, Biopharmaceuticals, cell, cells, Chinese, Cycle, Efficiency, Hamster, Ovary, study, Transfection | Posted under Transfection Books | No Comments