Monday, February 06, 2012

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Does anyone know of a good method for transfection of HEK 293 cells?

Question by Scooter: Does anyone know of a good method for transfection of HEK 293 cells?
I have been transfecting HEK 293 cells with Lipofectamine and I lose 90-100% of the cells after transfection. I’m trying to find a way to transfect and get the results I want with minimal cell death.

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