Posts Tagged ‘Stable’
Last Updated on Saturday, 7 August 2010 07:35 Written by Administrator Saturday, 7 August 2010 07:35
Question by AndroG: Hi, does anyone know how many weeks/months is the eluted DNA stable in a Tris-Cl,EDTA, pH8 solution at 4dgr?
To be more precized, I extracted the plasmid with my gene of interest from a bacteria with the special maxi kit endo-free from Qiagen and I eluted it with a buffer which contains 10mM Tris-C,pH8.0 and 1mM EDTA.I want to know how long can I store the eluted DNA at 4 degrees before using it for transfection into cells. Is DNA stable for several weeks or months? I would appreciate very much your answer as I am just a PhD student with not so much experience in molecular biology. Thank u very much.
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Tags: 4dgr, anyone, eluted, know, many, solution, Stable, TrisClEDTA, weeks/months | Posted under Transfection Questions | No Comments
Last Updated on Sunday, 11 July 2010 06:26 Written by Administrator Sunday, 11 July 2010 06:26
Science Magazine 14 December 1990: Homologous Recombination and Stable Transfection in the Parasitic Protozoan
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Tags: 1990, December, Homologous, Magazine, Parasitic, Protozoan, Recombination, Science, Stable, Transfection | Posted under Transfection Books | No Comments