What is the proper technique to gain a stabily transfected cell line using Lipofectamine 2000 on HeLa cells?
What is the proper technique to gain a stabily transfected cell line using Lipofectamine 2000 on HeLa cells?
Last Updated on Thursday, 3 June 2010 03:25 Written by Administrator Thursday, 3 June 2010 03:25
Question by benji: What is the proper technique to gain a stabily transfected cell line using Lipofectamine 2000 on HeLa cells?
I know the general scheme, but wanted more specifics of how long to treat with reagent of choice and when to split when selecting.
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Tags: 2000, cell, cells, gain, HeLa, line, Lipofectamine, proper, stabily, technique, transfected, using